研究業績（論文、特許、その他） 向井秀仁
注）責任著者である場合*を付しました。
論文
1. Munekata, E., Okada, M., Ishijima, T., Mukai, H., Goto, K., and Kanazawa, I.:
Correlations between structure and activity of neuromedin B isolated from porcine spinal cord (II),
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1984, 301-304, (1985).
2. Mukai, H., Tanaka, H., Osakada, F., Chiba, Y., Kawai, K., Yamashita, K., and Munekata, E.:
Structure-activity relationships of bombesin like neuropeptides (III),
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1985, 297-300, (1986).
3. Tanaka, H., Osakada, F., Kubo, K., Mukai, H., Muramatsu, R., Munekata, E., Wakamatsu, K., Miyazawa, T., Ohashi, S., and Shiraki, M.:
Studies on the interaction of neuropeptides and phospholipid membrane,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1985, 311-316, (1986).
4. *Mukai, H., Kawai, K., Suzuki, Y., Yamashita, K., and Munekata, E.:
Stimulation of dog gastropancreatic hormone release by neuromedin B and its analogues,
Am. J. Physiol., 252, E765-E771, (1987).
5. 川井紘一，向井秀仁，千葉之宣，宗像英輔，山下亀次郎：
Neuromedin BおよびGRP-10の膵 hormone分泌刺激作用，
PEPTIDE HORMONES IN PANCREAS，7, 335-341, (1987).
6. Kawai, K., Chiba, Y., Mukai, H., Munekata, E., and Yamashita, K.:
Effects of neuromedin B, gastrin-releasing peptide-10 and their fragment peptides on secretion of gastrointestinal and pancreatic hormones in dogs,
Acta Endocrinol., 117, 205-213, (1988).
7. Kawai, K., Suzuki, S., Murayama, Y., Mukai, H., Ohashi, S., and Yamashita, K.:
The biological effects of glucagon-like peptide-1 (GLP-1) and its structure activity,
Biomed. Res., 9 (suppl. 3), 213-217, (1988).
8. Mukai, H., Kawai, K., Yamashita, K., and Munekata, E.:
Stimulation of dog gastro-pancreatic hormone release by neuromedin B, GRP-10 and their derivatives,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1987, 463-468, (1988).
9. *Mukai, H., Kawai, K., Suzuki, S., Ohmori, H., Yamashita, K., and Munekata, E.:
[Ala6]gastrin-releasing peptide-10: an analogue with dissociated biological activities,
Am. J. Physiol., 257, E235-E240, (1989).
10. Kawai, K., Mukai, H., Chiba, Y., Ohmori, H., Suzuki, S., Munekata, E., and Yamashita, K.:
Effects of neuromedin B on insulin and glucagon release from the isolated perused rat pancreas,
Endocrinol. Japon., 36, 587-594, (1989).
11. Kawai, K., Suzuki, S., Ohashi, S., Mukai, H., Ohmori, H., Murayama, K., and Yamashita, K.:
Comparison of the effects of glucagon-like peptide-1 (1-37) and -(7-37) and glucagon on islet hormone release from the isolated perfused canine and rat pancreas,
Endocrinology, 124, 1768-1773, (1989).
12. Suzuki, S., Kawai, K., Ohashi, S., Mukai, H., and Yamashita, K.:
Comparison of the effect of various C-terminal and N-terminal fragment peptides of glucagon-like peptide-1 (GLP-1) on insulin and glucagon release from the isolated perfused rat pancreas,
Endocrinology, 125, 3109-3114, (1989).
13. Mukai, H., Kawai, K., Suzuki, S., Yamashita, K., and Munekata, E.:
Study on the antagonist peptide of bombesin-like neuropeptide,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1988, 61-66, (1989).
14. 鈴木誠司，川井紘一，大箸信一，向井秀仁，宗像英輔，山下亀次郎：
Truncated GLP-1の膵ホルモン分泌作用へのＣ末端アミノ酸残基の影響,
PEPTIDE HORMONES IN PANCREAS，9, 140-145, (1989).
15. Mukai, H., Kawai, K., Suzuki, S., Yamashita, K., and Munekata, E.:
Antagonism by GRP(18-27) and substance P analogs on insulin release stimulated by GRP(18-27),
Peptides, 11, 173-175, (1990).
16. Takuwa, N., Takuwa, Y., Ohue, Y., Mukai, H., Endo, E., Yamashita, K., and Munekata, E.:
Stimulation of calcium mobilization but not proliferation by bombesin and tachykinin neuropeptides in human small cell lung cancer cells,
Cancer Res., 50, 240-244, (1990).
17. Kawai, K., Mukai, H., Yuzawa, Y., Suzuki, S., Kuzuya, N., Fujii, K., Munekata, E.,
and Yamashita, K.:
Effect of neuromedin B and GRP-10 on gastrin and insulin release from cultured tumor cells of malignant gastrinoma,
Endocrinol. Japon., 37, 857-865, (1990).
18. Suzuki, S., Kawai, K., Ohashi, S., Mukai, H., Murayama, K., and Yamashita, K.:
Reduced insulinotropic effects of glucagon like peptide 1-(7-36)-amide and gastric inhibitory polypeptide in isolated perfused diabetic rat pancreas,
Diabetes, 39, 1320-1325, (1990).
19. Kawai, K., Suzuki, S., Ohashi, S., Mukai, H., Murayama, K., and Yamashita, K.:
Effect of truncated glucagon-like peptide-1 on pancreatic hormone release in normal conscious dogs,
Acta Endocrinol., 123, 661-667, (1990).
20. Mukai, H., Kawai, K., Suzuki, S., Yamashita, K., and Munekata, E.:
Antagonist of gastrin-releasing peptide,
Peptides (Proceedings of American Peptide Symposium), 1989, 171-173, (1990).
21. Ohashi, S., Suzuki, S., Mukai, H., Kawai, K., and Yamashita, K.:
Synthesis and biological activities of glucagon like peptide-1 (GLP-1) related peptides,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1989, 75-80, (1990).
22. 向井秀仁，宗像英輔：
ボンベシン群ペプチド，
化学と生物，28, 152-161, (1990).
23. 鈴木誠司，川井紘一，大箸信一，向井秀仁，村山耕子，山下亀次郎：
Truncated GLP-1のインスリン分泌刺激作用に対するアンタゴニストの開発,
PEPTIDE HORMONES IN PANCREAS，10, 188-192, (1990).
24. Mukai, H., Tanaka, H., Goto, K., Kawai, K., Yamashita, K., and Munekata, E.:
Structure-activity relationships of mammalian bombesin-like neuropeptides
in the contraction on the rat uterus,
Neuropeptides, 19, 243-250, (1991).
25. *Mukai, H., Munekata, E., and Higashijima, T.:
G protein antagonist - A novel hydrophobic peptide competes with receptor for G protein binding,
J. Biol. Chem., 267, 16237-16243, (1992).
26. *Mukai, H., Abe, Y., Munekata, E., and Higashijima, T.:
The regulation of G protein by substance P-related peptide: inhibition of the effect of mastoparan and receptor,
Peptides (Proceedings of American Peptide Symposium), 1991, 466-467, (1992).
27. Vitale, N., Mukai, H., Rouot, B., Thierse, D., Aunis, D., and Bader, M.-F.:
Exocytosis in chromaffin cells: possible involvement of the heterotrimeric GTP-binding protein Go,
J. Biol. Chem., 268, 14715-14723, (1993).
28. Mukai, H., and Higashijima, T.:
Inhibitory effects of substance P analogs on the activation of GTP-binding regulatory proteins by receptors,
Regl. Pept., 46, 338-339, (1993).
29. *Mukai, H., Munekata, E., and Higashijima, T.:
Antagonists of GTP-binding regulatory proteins (G proteins): substance P-related peptides compete with receptor for G protein binding,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1992, 330-333, (1993).
30. *Mukai, H., and Higashijima, T.:
The regulatory mechanisms of GTP-binding regulatory proteins by mastoparan and its derivatives,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1993, 309-312, (1994).
31. Fukuhara, S., Mukai, H., and Munekata, E.:
Pharmacological evidence for neurokinin receptors in murine neuroblastoma C1300 cells,
Peptides, 16, 211-214, (1995).
32. Fukuhara, S., Mukai, H., and Munekata, E.:
Intracellular signal transduction induced by neurokinin receptors in C1300 cells,
Peptides (Proceedings of European Peptide Symposium), 1994, 775-776, (1995).
33. Fukuhara, S., Shimizu, M., Mukai, H., and Munekata, E.:
Mechanisms of amylase secretion induced by neurokinins in AR42J rat pancreatic acinar cells,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1994, 385-388, (1995).
34. Fukuhara, S., Mukai, H., Kako, K., Nakayama, K., and Munekata, E.:
Further identification of neurokinin receptor types and mechanisms of calcium signaling evoked by neurokinins in the murine neuroblastoma C1300 cell line,
J. Neurochem., 67, 1282-1292, (1996).
35. Mukai, H., Higashijima, T., Suzuki, Y., and Munekata, E.:
The regulation of GTP-binding regulatory proteins by substance P, mastoparan
and their derivatives,
Peptides (Proceedings of American Peptide Symposium), 1995, 403-405, (1996).
36. Fukuhara, S., *Mukai, H., Shimizu, M., and Munekata, E.:
Intracellular signal transduction involved in neurokinin receptors,
Peptides (Proceedings of American Peptide Symposium), 1995, 410-411, (1996).
37. Abe, J., Fujinaka, H., Mukai, H., and Munekata, E.:
Study on the activation mechanism of neurokinin receptors,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1995, 277-280, (1996).
38. Fukuhara, S., Mukai, H., and Munekata, E.:
Activin A and all-trans-retinoic acid cooperatively enhanced the functional
activity of L-type Ca2+ channels in the neuroblastoma C1300 cell line,
Biochem. Biophys. Res. Commun., 241, 363-368, (1997).
39. Fukuhara, S., Mukai, H., and Munekata, E.:
Identification of neurokinin receptor types and intracellular signal transduction
induced by them in the murine neuroblastoma C1300 cell line,
Peptide Chemistry (Proceedings of Japanese Symposium on Peptide Chemistry), 1996, 289-292, (1997).
40. Fukuhara, S., Shimizu, M., Matsushima, H., Mukai, H., and Munekata, E.:
Signaling pathways via NK1 receptors and their desensitization in an AR42J cell line,
Peptides, 19, 1349-1357, (1998).
41. Tanaka, T., Kohno, T., Kinoshita, S., Mukai, H., Itoh, H., Ohya, M., Miyazawa, T., Higashijima, T., and Wakamatsu, K.:
Helix content of G protein a subunit is decreased upon activation by receptor
mimetics,
J. Biol. Chem., 273, 3247-3252, (1998).
42. Mukai, H., Kikuchi, M., Suzuki, Y., and Munekata, E.:
The mechanisms of exocytosis induced by neurokinin peptides in mast cells,
Peptide Science (Proceedings of Japanese Peptide Symposium), 1997, 179-182, (1998).
43. Fukuhara, S., Mukai, H., and Munekata, E.:
Charcterization of neurokinin receptors types and their intracellular signaling in C1300 cells,
Peptides (Proceedings of American Peptide Symposium), 1997, 648-649, (1998).
44. Shin, H.-W., Takatsu, H., Mukai, H., Munekata, E., Murakami, K., and Nakayama, K.:
Intermolecular and interdomain interaction of a dynamin-related GTP-binding protein,
Dnp1p/Vps1p-like protein,
J. Biol. Chem., 274, 2780-2785, (1999).
45. Aoki, Y., Sha, S., Mukai, H., and Nishi, Y.:
Selective stimulation of G-CSF in gene expression macrophages by a stimulatory monoclonal antibody as detected by a luciferase reporter gene assay,
J. Leukoc. Biol., 68, 757-764, (2000).
46. Nakajima, T., Wakamatsu, K., and Mukai, H.:
Mastoparan as a G protein-activator,
Methods and Tools in Biosciences and Medicine, Animal Toxins (Rochat, H.,
and Martin-Eauclaire, M.-F. eds.), 116-126, Switzerland, Birkhauser (2000).
47. *Mukai, H., Hokari, Y., Seki, T., Nakano, H., Takao, T., Shimonishi, Y., Nishi, Y.,
and Munekata, E.:
Novel classes of neutrophil-activating peptides: Isolation and their physiological significance,
Peptides (Proceedings of American Peptide Symposium), 2001, 1014-1015, (2001).
48. *向井秀仁，若松馨：
Ｇ蛋白質アクチベータとしてのスズメバチ毒、マストパラン，
蛋白質・核酸・酵素，46, 423-428, (2001).
49. *向井秀仁：
新たな生体調節物質同定の試み−タンパク質”第二の機能”と関連して，
ペプチドニュースレター，41, 1-2, (2001).
50. *Mukai, H., Aoki, Y., Sha, S., and Nishi, Y.:
A stimulatory monoclonal antibody that induces G-CSF release from macrophages,
Peptides (Proceedings of European Peptide Symposium), 2002, 990-991, (2002).
51. *Mukai, H., Matsuo, Y., Kamijo, R., and Wakamatsu, K.:
Novel neutrophil-activating peptides: physiological roles of direct activation of GTP-binding regulatory proteins by these peptides,
Peptide Revolution: Genomics, Proteomics and Therapeutics (Proceedings of American Peptide Symposium), 2003, 553-555, (2004).
52. Yabuki, Y., Muramatsu, T., Hirokawa, T., Mukai, H., and Suwa, M.:
GRIFFIN: a system for GPCR-G-protein coupling selectively using a support vector machine and a hidden Markov model,
Nucleic Acids Res., 33, W148-W153, (2005).
53. *Mukai, H., Someya, K., Matsuo, Y., Kamijo, R., and Wakamatsu, K.:
Functional cryptic peptides: identification of bioactive peptides hidden in protein structures,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2004, 103-106, (2005).
54. Someya, K., Matsuo, Y., Kamijo, R., Hojo, Y., Nakahara, Y., and *Mukai, H.:
Structure-activity relationships of fCyt b related peptides, functional cryptic peptides that activate neutrophils,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2004, 521-522, (2005).
55. *Mukai, H., Someya, K., Ueki, N., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Prediction and identification of endogenous functional cryptic peptides
that directly activate G proteins,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2005, 207-208, (2006).
56. *Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Functional cryptic peptides and their accumulative signaling,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2006, 152, (2006).
57. Kawanami, M., Someya, K., Ueki, N., Kiyama, M., and *Mukai, H.:
Structural requirements of mitocryptide-2 (fCyt b) for the binding and
the activation of its receptors,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2006, 173, (2006).
58. Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and *Mukai, H.:
Identification of receptors for mitocryptides that activate neutrophils,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2006, 308, (2006).
59. 植木暢彦，*向井秀仁：
クリプティック機能ペプチド-系統的同定と新たな情報伝達系accumulative signaling,
化学と生物 44, 728-730, (2006).
60. Ueki, N., Someya, K., Matsuo, Y., Wakamatsu, K., and *Mukai, H.:
Cryptides: functional cryptic peptides hidden in protein structures,
Biopolymers (Pep. Sci.), 88, 190-198, (2007).
61. Asada, S., Daitoku, H., Matsuzaki, H., Saito, T., Sudo, T., Mukai,
H., Iwashita, S., Kako, K., Kishi, T., Kasuya, Y., and Fukamizu, A.:
Mitogen-activated protein kinases, Erk and p38, phosphorylate and regulate Foxo1,
Cell. Signal., 19, 519-527, (2007).
62. *Mukai, H., Kikuchi, M., Suzuki, Y., and Munekata, E.:
A mastoparan analog without lytic effects and its stimulatory mechanisms in mast cells,
Biochem. Biophys. Res. Commun., 362, 51-55, (2007).
63. *Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Functional cryptic peptides and their physiological roles,
Peptides (Proceedings of European Peptide Symposium), 2006, 746-747, (2007).
64. *Mukai, H., Kikuchi, M., Fukuhara, S., Kiso, Y., and Munekata, E.:
Cryptide signaling: amphiphilic peptide-induced exocytotic mechanisms in mast cells,
Biochem. Biophys. Res. Commun., 375, 22–26, (2008).
65. *Mukai, H., Suzuki, Y., Kiso, Y., and Munekata, E.:
Elucidation of structural requirements of mastoparan for mast cell activation –
Toward the comprehensive prediction of cryptides acting on mast cells,
Protein Pept. Lett., 15, 931–937, (2008).
66. *Mukai, H., Hokari, Y., Seki, T., Takao, T., Kubota, M., Matsuo, Y.,
Tsukagoshi, H., Kato, M., Kimura, H., Shimonishi, Y., Kiso, Y., Nishi,
Y., Wakamatsu, K., and Munekata, E.:
Discovery of mitocryptide-1, a neutrophil-activating cryptide from healthy porcine heart,
J. Biol. Chem., 283, 30596–30605, (2008).
67. Yamagata, K., Daitoku, H., Takahashi, Y., Namiki, K., Hisatake, K., Kako, K.,
Mukai, H., Kasuya, Y., and Fukamizu, A.:
Arginine methylation of FOXO transcription factors inhibits their phospholylation by Akt,
Mol. Cell, 32, 221–231, (2008).
68. Taniguchi, A., Skwarczynski, M., Sohma, Y., Okada, T., Ikeda, K., Prakash, H.,
Mukai, H., Hayashi, Y., Kimura, T., Hirota, S., Matsuzaki, K., and Kiso, Y.:
Controlled production of amyloid b peptide from a photo-triggered, water-soluble
precursor "click peptide",
ChemBioChem, 9, 3055–3065, (2008).
69. *Mukai, H., and Wakamatsu, K.:
Cryptides: functional cryptic peptides hidden in protein structures and
their novel signaling mechanisms,
Proceedings of 4th International Peptide Symposium, Published on line (2008); http://peptideoz.org/docs/L_338_Hidehito_Mukai.pdf
70. *Mukai, H., Ueki, N., Kawanami, M., Kiyama, M., Kiso, Y., and Wakamatsu, K.:
Investigation of signaling mechanisms induced by mitocryptides in neutrophilic/granulocytic cells,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2007, 375-376, (2008).
71. Taniguchi, A., Sohma, Y., Hirayama, Y., Mukai, H., Kimura, T., Hayashi,
Y., Matsuzaki, K., and Kiso, Y.:
"Click peptide": pH-triggered in situ production and aggregation of monomer Ab1-42,
ChemBioChem, 10, 710–715, (2009).
72. *Mukai, H., Seki, T., Nakano, H., Hokari, Y., Takao, T., Kawanami, M., Tsukagoshi, H., Kimura, H., Kiso, Y., Shimonishi, Y., Nishi, Y., and Munekata, E.:
Mitocryptide-2: purification, identification and characterization of a novel cryptide that activates neutrophils,
J. Immunol., 182, 5072–5080, (2009).
73. Taniguchi, A., Sohma, Y., Skwarczynski, M., Nagano, T., Okada, T.,
Ikeda, K., Prakash, H., Mukai, H., Hayashi, Y., Kimura, T., Hirota, S.,
Matsuzaki, K., and Kiso, Y.:
Development of water-soluble click peptides by use of the O-acyl isopeptide method: In situ production of Alzheimer’s amyloid b peptide,
Peptides (Proceedings of European Peptide Symposium), 2008, 32–33, (2009).
74. *Mukai, H., Ueki, N., Wakamatsu, K., and Kiso, Y.:
Cryptide signal: a novel signaling mechanisms involving cryptides,
Peptides (Proceedings of European Peptide Symposium), 2008, 570–571, (2009).
75. Taniguchi, A., Sohma, Y., Skwarczynski, M., Okada, T., Ikeda, K., Prakash, H.,
Mukai, H., Kimura, T., Hayashi, Y., Hirota, S., Matsuzaki, K., and Kiso, Y.:
Click peptide by use of the O-acyl isopeptide method: production of amyloid b peptide from water-soluble analogue,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2008, 121–122, (2009).
76. *Mukai, H., Ueki, N., Someya, K., Kawanami, M., Matsuo, Y., Kiyama, M., Kamijo, R.,
Kikichi, M., Fukuhara, S., Munekata, E., and Kiso, Y.:
Cryptide signaling and exocytotic mechanisms induced by amphiphilic peptides,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2008, 307–310, (2009).
77. Wakamatsu, K., Takei, A., Inaoka, Y., Xian, L., Kobori, K., Kato, S.,
Mukai, H., Kohno, T., and Nameki, N.:
Prevention of peptide aggregation by non-detergent sulfobetaines (NDSBs),
Peptide Science (Proceedings of Japanese Peptide Symposium), 2008, 397–400, (2009).
78. Taniguchi, A., Sohma, Y., Skwarczynski, M., Hirayama, Y., Okada, T., Ikeda, K., Prakash, H., Mukai, H., Kimura, T., Hayashi, Y., Hirota, S., Matsuzaki, K., and Kiso, Y.:
"Click peptide" based on "O-acyl isopeptide method": In situ production of monomer amyloid b Peptide from water-soluble precursor analogues,
Peptides: Break Away (Proceedings of American Peptide Symposium), 2009, 195–196, (2010).
79. *Mukai, H., Seki, T., Munekata, E., and Kiso, Y.:
Cryptides: Discovery of functional cryptic peptides hidden in protein structures and identification of their signaling mechanisms,
Peptides: Break Away (Proceedings of American Peptide Symposium), 2009, 203–204, (2010).
80. *Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Characterization of receptors and signaling mechanisms for mitocryptide-2, a novel cryptide that activates neutrophils,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2009, 97–98, (2010).
81. Wang, H., Kakizawa, T., Taniguchi, A., Mizuguchi, T., Sohma, Y., Mukai, H., Kimura, T.,
and Kiso, Y.:
Investigation of Ab1–42 mutants based on O-acyl isopeptide method; Synthesis of O-acyl isopeptides and in situ production of their native forms,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2009, 367–368, (2010).
82. Hirayama, Y., Taniguchi, A., Sohma, Y., Mukai, H., Kimura, T., Hayashi, Y., Matsuzaki, K., and Kiso, Y.:
Click peptide: Water-soluble precursor producing monomer amyloid b peptide in situ,
Peptide Science (Proceedings of Japanese Peptide Symposium), 2009, 371–372, (2010).
83. *Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Cryptides: receptors and signaling mechanisms for novel neutrophil-activating peptides hidden in mitochondrial proteins,
Peptides (Proceedings of European Peptide Symposium), 2010, 36–37, (2010).
84. *向井秀仁，木曽良明，若松馨：
クリプタイドの発見と新しい生体情報伝達機構,
生化学 82, 524-532, (2010).
85. Seki, T., Fukamizu, A., Kiso, Y., and *Mukai, H.:
Mitocryptide-2, a neutrophil-activating cryptide, is a specific endogenous agonist for fromyl-peptide receptor-like 1,
Biochem. Biophys. Res. Commun., 404, 482–487, (2011).
86. Sohma, Y., Hirayama, Y., Taniguchi, A., Mukai, H., and Kiso, Y.:
'Click peptide' using production of monomer Ab from the O-acyl isopeptide: Application to assay system of aggregation inhibitors and cellular cytotoxicity,
Bioorg. Med. Chem., 19, 1729–1733, (2011).
87. Sohma, Y., Wang, H., Taniguchi, A., Hirayama, Y., Kakizawa, T., Yamazaki, M.,
Mukai, H., and Kiso, Y.:
Self-assembly pathways of E22D-type amyloid b peptide mutants generated from non-aggregative O-acyl isopeptide precursors,
Bioorg. Med. Chem., 19, 3787–3792, (2011).
88. *Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Cryptides: non-classical bioactive peptides hidden in protein structures,
Peptide Science (Proceedings of 5th International Peptide Symposium), 2010, 29, (2011).
89. Sohma, Y., Taniguchi, A., Hirayama, Y., Wang, H., Yamazaki, M., Mukai, H., and Kiso, Y.:
Use of O-acyl isopeptides to identify new functions of amyloid b peptides,
Peptide Science (Proceedings of 5th International Peptide Symposium), 2010, 54, (2011).
90. Yamazaki, M., Sohma, Y., Taniguchi, A., Hirayama, Y., Furuya, M., Mukai, H., and Kiso, Y.:
Click peptide: In situ production of pyroGlu-Abeta from the O-acyl isopeptide,
Peptide Science (Proceedings of 5th International Peptide Symposium), 2010, 210, (2011).
91. Wang, H., Sohma, Y., Taniguchi, A., Hirayama, Y., Kakizawa, T., Mukai, H., and Kiso, Y.:
Click peptide: application to E22D-type mutant of amyloid b peptide,
Peptide Science (Proceedings of 5th International Peptide Symposium), 2010, 221, (2011).
92. *Mukai, H. and Kiso, Y.:
Cryptides and their accumulative signaling: a novel regulatory mechanism by functional peptides hidden in protein structures,
Peptides: Building Bridges (Proceedings of 22nd American Peptide Symposium), 2011, 314–315, (2011).
93. Hokari, Y., Seki, T., Nakano, H., Fukamizu, A., Munekata, E., Kiso, Y., and *Mukai, H.:
Novel neutrophil-activating cryptides hidden in mitochondrial cytochrome c: identification and structure-activity studies,
Peptide Science (Proceedings of 48th Japanese Peptide Symposium), 2011, 61–62, (2012).
94. Hokari, Y., Seki, T., Nakano, H., Matsuo, Y., Fukamizu, A., Munekata, E., Kiso, Y., and
*Mukai, H.:
Isolation and identification of novel neutrophil-activating cryptides hidden in mitochondrial cytochrome c,
Prot. Pept. Lett., 19, 680–687, (2012).
95. *向井秀仁，木曽良明：
クリプタイド：タンパク質に隠された新しい生理活性ペプチド−その発見と存在意義および効率的スクリーニング−,
遺伝子医学MOOK 21, 298-304, (2012).
96. *向井秀仁，木曽良明：
クリプタイド：タンパク質構造に隠された新しい生理活性ペプチドとその生体機能および創薬への適用,
ペプチド医薬の最前線，木曽良明、向井秀仁監修，シーエムシー出版，52-59, (2012).
97. Hokari, Y., Seki, T., Nakano, H., Matsuo, Y., Fukamizu, A., Munekata, E., Kiso, Y.,
and *Mukai, H.:
Novel neutrophil-activating cryptides hidden in mitochondrial cytochrome c,
Peptides (Proceedings of 32nd European Peptide Symposium), 2012, 310–311, (2012).
98. *向井秀仁，木曽良明：
クリプタイド：タンパク質構造に隠された新しい生理活性ペプチド，
比較内分泌学，38, 196-202, (2012).
99. Sohma, Y., Yamazaki, M., Kawashima, H., Taniguchi, A., Yamashita, M., Akaji, K., , Mukai, H., and Kiso, Y.:
Comparative properties of Aβ1–42, Aβ11–42, and [Pyr11]Aβ11–42 generated from O-acyl isopeptides,
Bioorg. Med. Chem. Lett., 23, 1326-1329, (2013).
100. *向井秀仁，木曽良明：
クリプタイド：タンパク質に隠された新しい機能ペプチド，
バイオサイエンスとインダストリー，71, 18-22, (2013).
101. Hattori, T., Shirota, S., Marutani, T., Kiso, Y., Nishi, Y., and Mukai, H.:
Functional analysis of mitocryptides with their monoclonal antibodies,
Peptide Science (Proceedings of 49th Japanese Peptide Symposium), 2012, 277–278, (2013).
102. Kawashima, H., Sohma, Y., Nakanishi, T., Kitamura, H., Mukai, H., Yamashita, M., Akaji, K., and Kiso, Y.:
A new class of aggregation inhibitor of amyloid-b peptide based on an O-acyl isopeptide,
Bioorg. Med. Chem., 21, 6323-6327, (2013).
103. *Mukai, H., Hokari, Y., Seki, T., Fukamizu, A., and Kiso, Y.:
Identification and structure-activity studies of novel cryptides hidden in mitochondrial proteins,
Peptides (Proceedings of 23rd American Peptide Symposium), 2013, 314–315, (2013).
特許
1. 向井秀仁，西義介，宗像英輔：
好中球刺激活性を有するポリペプチド，
特許願2000-62045，(2000) およびPCT出願（公開番号：WO0166734）.
2. 謝志堅，向井秀仁，青木良子，西義介：
新規タンパク質、それをコードする遺伝子、及びそれらの利用方法，
特許願2000-294191，(2000)およびPCT出願（公開番号：WO0226978）.
3. 諏訪牧子、広川貴次、向井秀仁：
活性化Ｇ蛋白質予測装置、プログラムおよび方法，
特許願2004-316279，(2004).
4. 諏訪牧子、広川貴次、向井秀仁：
Ｇ蛋白質共役受容体を活性化するリガンドの同定およびその方法，
特許願2006-038503，(2006)およびPCT出願（公開番号：WO2007094330）.
5. 向井秀仁、若松 馨：
免疫細胞を活性化する内因性機能ペプチド，
特許願2006-267559，(2006)およびPCT出願（公開番号：WO2008038766）.
その他
国際学会招待・依頼講演
1. Mukai, H., Munekata, E., and Higasijima, T.:
Antagonist of GTP-binding regulatory proteins (G proteins),
2nd Japan Symposium on Peptide Chemistry, 1992年11月, 静岡
2. Mukai, H., Higashijima, T., Suzuki, Y., and Munekata, E.:
The regulation of GTP-binding regulatory proteins by substance P, mastoparan
and their derivatives,
14th American Peptide Symposium, 1995年6月, Columbus, USA
3. Mukai, H., Kikuchi, M., Suzuki, Y., and Munekata, E.:
The mechanisms of exocytosis induced by neurokinin peptides in mast cells,
1st International Peptide Symposium, 1997年12月, 京都
4. Mukai, H., Matsuo, Y., Kamijo, R., and Wakamatsu, K.:
Novel neutrophil-activating peptides: physiological roles of direct activation
of GTP-binding regulatory proteins by these peptides,
18th American Peptide Symposium, 2003年7月, Boston, USA
5. Mukai, H., Someya, K., Matsuo, Y., Kamijo, R., and Wakamatsu, K.:
Functional cryptic peptides: identification of bioactive peptides hidden in protein structures,
1st Asia-Pacific International Peptide Symposium, 2004年11月, 福岡
6. Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Functional cryptic peptides and their accumulative signaling mechanisms,
International Conference of 43rd Japanese Peptide Symposium / 4th Peptide Engineering Meeting,
2006年11月，横浜 （招聘講演）
7. Mukai, H.:
Cryptides: functional cryptic peptides hidden in protein structures,
7th International Symposium on Peptide and Proteineous Materials, 2007年4月,
Gwangju, Korea （基調講演）
8. Mukai, H., and Wakamatsu, K.:
Cryptides: functional cryptic peptides hidden in protein structures and their novel signaling
mechanisms,
4th International Peptide Symposium, 2007年10月, Cairns, Australia （基調講演）
9. Mukai, H., Wakamatsu, K., and Kiso, Y.:
Cryptides: functional cryptic peptides hidden in protein structures – Discovery and
their physiological roles –,
The 12th Akabori Conference (German-Japanese Symposium on Peptide Science),
2008年5月，京都 （招待講演）
10. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Cryptides: functional peptides hidden in protein structures - Discovery and physiological significance,
3rd Asia-Pacific International Peptide Symposium, 2009年11月，Jeju, Korea（招待講演）
11. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Clarification of signaling mechanisms for mitocryptides, novel neutrophil-activating peptides hidden in mitochondrial proteins,
12th Naples Workshop on Bioactive Peptides, 2010年6月，Naples, Italy
12. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Mitocryptides: a novel family of neutrophil-activating peptides derived from mitochondrial proteins and their regulatory mechanisms,
The 11th Chinese International Peptide Symposium, 2010年7月，Lanzhou, China （招待講演）
13. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Cryptides: receptors and signaling mechanisms for novel neutrophil-activating peptides hidden in mitochondrial proteins,
31st European Peptide Symposium, 2010年9月, Copenhagen, Denmark
14. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Mitocryptides: novel neutrophil-activating cryptides hidden in mitochondrial proteins,
The 13th Akabori Conference (German-Japanese Symposium on Peptide Science), 2010年
9月, Leipzig, Germany （招待講演）
15. Mukai, H., and Kiso, Y.:
Cryptides: Non-classical bioactive peptides hidden in protein structures,
5th International Peptide Symposium, 2010年12月，京都 （招待講演）
16. Mukai, H. and Kiso, Y.:
Cryptides and their accumulative signaling: a novel regulatory mechanism by functional peptides hidden in protein structures,
22nd American Peptide Symposium, 2011年6月，San Diego, USA （招聘講演）
17. Mukai, H., Hokari, Y., Seki, T., Fukamizu, A., and Kiso, Y.:
Cryptides: Functional peptides hidden in protein structures and their possible applications,
9th Australian Peptide Conference, 2011年10月，Hamilton Island, Australia （招待講演）
18. Mukai, H.:
Mitocryptides: a novel family of neutrophil-activating peptides hidden in mitochondrial proteins and their accumulative signaling mechanisms,
International Conference on Biothreats and Biodefense 2012, 2012年10月，Chicago, USA
（招待講演）
19. Mukai, H., Hokari, Y., Seki, T., Fukamizu, A., and Kiso, Y.:
Cryptides: identification of novel bioactive peptides hidden within protein sequences,
Peptide Chemistry Conference 2012, 2012年11月，Xcaret, Mexico（招待講演）
シンポジウム、セミナー、国内学会等における招待講演
1. 向井秀仁：
炎症とペプチド-情報伝達性ペプチドによるＧタンパク質直接的活性化の生理的意義，
第33回若手ペプチド夏の勉強会, 2000年7月, 清水
2. 向井秀仁：
新たな内因性活性化物質検索の試み−新たな好中球活性化因子の単離・同定と機能解析−，
三菱化学生命科学研究所, 2002年2月
3. 向井秀仁：
生体調節ペプチド探索の現状と今後の課題，
第3回ペプチドフォーラム, 2003年2月, 札幌
4. 向井秀仁：
新しい好中球活性化ペプチドとそれらが関与する疾病について，
川井クリニック研究セミナー, 2003年3月
5. 向井秀仁：
バイオインフォマティックス技術を用いた新たな生体調節ペプチドの探索，
国立遺伝学研究所・セミナー 研究集会「新時代のペプチドバイオロジー」，2003年3月
6. Mukai, H.:
Functional cryptic peptides: novel neutrophil-activating peptides and physiological roles of direct activation of G proteins by those peptides,
Research Seminar, Moffitt Cancer Institute, University of South Florida, 2003年7月
7. 向井秀仁：
ポストゲノム時代のペプチド研究〜世界を征する独創的研究領域開拓とそれを可能にする日本発の分析技術，
分析展2004, 2004年9月, 幕張
8. 向井秀仁：
クリプティック機能ペプチドの網羅的探索と新たな生体情報伝達機構，
慶應義塾大学COEワークショップ, 2004年11月
9. 向井秀仁：
クリプティック機能ペプチド：新しい生体情報伝達機構の解明，
群馬大学工学部セミナー, 2004年12月
10. 向井秀仁：
ペプチド研究の新たな進展 〜事業化を見据えた基礎研究について〜，
日本海ペプチドフォーラム, 2005年1月, 富山
11. 向井秀仁：
クリプティック機能ペプチドの網羅的探索と新たな生体情報伝達機構，
サントリー生物有機科学研究所セミナー, 2005年2月
12. 向井秀仁：
クリプティック機能ペプチドの探索とaccumulative signaling，
国立循環器病センター研究所セミナー, 2005年7月
13. 向井秀仁：
クリプティック機能ペプチドの網羅的ならびに系統的探索と新たな生体情報伝達機構，
東京工業大学セミナー, 2005年7月
14. Mukai, H., Someya, K., Ueki, N., Matsuo, Y., Kubota, M., and Wakamatsu, K.:
Functional cryptic peptides: identification of neutrophil-activating peptides
hidden in protein structures,
第78回日本生化学会大会シンポジウム, 2005年10月 （招待講演）
15. 向井秀仁：
クリプティック機能ペプチドの系統的探索と新たな生体情報伝達機構，
産業技術総合研究所生命情報科学研究センターセミナー，2006年12月
16. 向井秀仁：
ペプチド研究における新世界の開拓：ケミカルバイオロジーとバイオインフォマティックスの融合，
NECバイオＩＴセミナー, 2006年1月
17. 向井秀仁：
ケミカルバイオロジーとバイオインフォマティックスの融合による機能ペプチドネットワーク解析，
筑波大学TARAセンターCOEセミナー，2006年10月
18. 向井秀仁：
ペプチド研究における新世界の開拓，
日本分子生物学会2006フォーラム，シンポジウム「ゲノムネットワーク解析に基づく新しい創薬、診断、治療戦略」，2006年12月 （招待講演）
19. 向井秀仁、若松馨、木曽良明：
クリプタイド（Cryptide）〜タンパク質配列に隠された機能ペプチドの発見とそれらの生体機能，
難病克服をめざした分子統合創薬科学研究成果発表会，2008年1月
20. 向井秀仁、若松馨、木曽良明：
タンパク質配列に隠された機能ペプチド、クリプタイドの発見とそれらの生理的機能，
日本ヒトプロテオーム機構第６回大会シンポジウム「ペプチドミクスによるバイオマーカー、活性ペプチドの探索」，2008年7月 （招待講演、英語）
21. 向井秀仁：
タンパク質配列に隠された内因性機能ペプチド、「クリプタイド（Cryptide）」，
第11回生命化学研究会〜生命化学をシステムで捉えたら〜，2008年11月 （招待講演）
22. 向井秀仁：
クリプタイドの網羅的同定と新しい生体調節機構解明への挑戦，
第12回ペプチドフォーラム，九州工業大学，2009年1月（招待講演）
23. 向井秀仁、関哲郎、保苅義則、深水昭吉、木曽良明：
タンパク質構造に隠された生理活性ペプチド、「クリプタイド」とその情報伝達機構「accumulative signaling」，
第82回日本生化学会大会シンポジウム「ペプチドの多様性と機能」，2009年10月，神戸（招待講演）
24. Mukai, H. and Kiso, Y.:
Discovery of cryptides and their physiological roles,
Seminor, Shenyang Pharmaceutical University, 2010年7月
25. 向井秀仁：
タンパク質に隠された新しい生理活性ペプチド、「クリプタイド（Cryptide）」 〜同定と生理機能〜，
大阪大学蛋白質研究所セミナー 「ペプチド研究の新潮流：発見から応用へ」，2011年3月
26. 向井秀仁：
新しい一群の生理活性ペプチド、クリプタイド（Cryptide）ーその発見と新しい生体調節機構ー，
長浜バイオ大学バイオセミナー，2011年4月
27. Mukai, H., Hokari, Y., Seki, T., Fukamizu, A., and Kiso, Y.:
Mitocryptides: functional cryptic peptides hidden in mitochondrial protein structures and their possible applications,
14th Peptide Forum, 2011年10月，鹿児島
28. 向井秀仁：
クリプタイド（Cryptides）：タンパク質に隠された新しいタイプの生理活性ペプチド，
第２回滋賀県ライフサイエンスセミナー，2012年1月，大津
29. 向井秀仁：
クリプタイド（Cryptides）：その存在意義と産業的応用，
第15回ペプチドフォーラム，2012年3月，長浜
30. 向井秀仁、木曽良明：
クリプタイド（Cryptides）：タンパク質構造に隠された新しい機能ペプチドの探索，
第５回GPCR研究会，2012年5月，東京（基調講演）
31. 向井秀仁：
拡大するペプチドワールド−その探索法と評価法，
第44回若手ペプチド夏の勉強会，2012年8月，大阪（テクニカルセミナー招待講演）
32. Mukai, H.:
Mitocryptides: a novel family of neutrophil-activating peptides hidden
in mitochondrial proteins and their accumulative signaling mechanisms,
Research Seminar, Department of Pharmacology, Pennsylvania State University
College of Medicine, 2012年10月，Pennsylvania, USA
33. 向井秀仁：
健康を守りいのちを育むペプチド〜栄養、機能食品から医薬まで
淡海生涯カレッジ，2012年10月，長浜
34. 向井秀仁：
クリプタイド：その存在意義と産業的応用
新科学技術推進協会ライフサイエンス技術部会・材料分科会講演会，2013年4月，東京
35. 向井秀仁：
クリプタイドと創薬−−タンパク質に隠された新しい生理活性ペプチド、クリプタイドの系統的探索および生体機能の解析と創薬への応用
生命分子機能研究会2013学術集会，2013年9月，長浜（招待講演）
国際学会一般口頭発表
1. Mukai, H., Hokari, Y., Seki, T., Nakano, H., Takao, T., Shimonishi, Y., Munekata, E., and Nishi, Y.:
Inflammatory activation of neutrophils triggered by a novel class of peptides,
2000 World Congress of In Vitro Biology, 2000年6月, San Diego, USA
2. Mukai, H. and Kiso, Y.:
Discovery of cryptides, functional cryptic peptides hidden in protein structures, and their accumulative signaling mechanisms,
International Symposium on Integrated Medicinal Science, 2008年12月, 京都
国際学会一般発表（本人が直接発表したもののみ）
1. Mukai, H., Kawai, K., Suzuki, S., Yamashita, K., and Munekata, E.:
Stimulation of dog gastro-pancreatic hormone release by neuromedin B, GRP-10 and their derivatives.
1st Japan Symposium on Peptide Chemistry, 1987年９月, 神戸
2. Mukai, H., Kawai, K., Suzuki, S., Yamashita, K., and Munekata, E.:
Antagonist of gastrin-releasing peptide.
11th American Peptide Symposium, 1989年7月, San Diego, USA
3. Mukai, H., Abe, Y., Munekata, E., and Higashijima, T.:
The regulation of G protein by substance P-related peptide: inhibition
of the effect of mastoparan and receptor,
12th American Peptide Symposium, 1991年6月, Boston, USA
4. Mukai, H., Abe, Y., Munekata E., and Higashijima, T.:
The regulation of G protein by substance P-related peptides,
Gordon Research Conference, 1991年6月, New Hampshire, USA
5. Mukai, H., and Higashijima, T:
Inhibitory effects of substance P analogs on the activation of GTP-binding
regulatory proteins by receptors,
International Symposium on Substance P and Related Peptides, 1992年11月, 静岡
6. Fukuhara, S., Mukai, H., and Munekata, E.:
Intracellular signal transduction induced by neurokinin receptors in C1300 cells,
23rd European Peptide Symposium, 1994年9月, Braga, Portugal
7. Mukai, H., Hokari, Y., Seki, T., Nakano, H., Takao, T., Shimonishi, Y., Nishi, Y., and Munekata, E.:
Novel classes of neutrophil activating peptides.
2nd International Peptide Symposium / 17th American Peptide Symposium,
2001年6月, San Diego, USA
8. Mukai, H., Aoki, Y., Sha, S., and Nishi, Y.:
A stimulatory monoclonal antibody that induces G-CSF release from macrophages,
27th European Peptide Symposium. 2002年9月, Sorrento, Italy
9. Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Identification and signaling mechanisms of cryptides, functional cryptic peptides hidden in protein structures,
20th IUBMB (International Union of Biochemistry and Molecular Biology) Congress,
2006年6月, 京都
10. Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Functional cryptic peptides and the physiological roles of them,
29th European Peptide Symposium, 2006年9月，Gdansk, Poland
11. Mukai, H., Ueki, N., Someya, K., Kawanami, M., Kiyama, M., and Wakamatsu, K.:
Identification of cryptides, functional cryptic peptides hidden in protein structures, by the combined investigation with bioinformatic, chemical and biological approaches,
20th American Peptide Symposium, 2007年6月, Montreal, Canada
12. Mukai, H., Wakamatsu, K. and Kiso, Y.:
Cryptide signal: a novel signaling mechanism involving cryptides,
30th European Peptide Symposium, 2008年9月, Helsinki, Finland
13. Mukai, H., Seki, T., Munekata, E., and Kiso, Y.:
Cryptides: discovery of functional cryptic peptides hidden in protein structures
and identification of their signaling mechanisms,
21st American Peptide Symposium, 2009年6月, Bloomington, USA
14. Mukai, H., Seki, T., Hokari, Y., Fukamizu, A., and Kiso, Y.:
Cryptides: discovery of functional cryptic peptides hidden in protein structures and investigation of their intracellular signaling mechanisms,
14th International Congress of Endocrinology, 2010年3月, 京都
15. Hokari, Y., Seki, T., Nakano, H., Matsuo, Y., Fukamizu, A., Munekata, E., Kiso, Y., and
Mukai, H.:
Novel neutrophil-activating cryptides hidden in mitochondrial cytochrome c,
32nd European Peptide Symposium, 2012年9月, Athens, Greece
16. Mukai, H., Hokari, Y., Seki, T., Fukamizu, A., and Kiso, Y.:
Identification and Structure-Activity Studies of Novel Cryptides Hidden in Mitochondrial Proteins,
6th International Peptide Symposium / 23rd American Peptide Symposium, 2013年6月，Hawaii, USA
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